Transduction efficacy of bone marrow-derived versus pulmonary macrophages in mice with either lentiviral or adenoviral vectors encoding for GM-CSF

Introduction: Granulocyte macrophage-colony stimulating factor (GM-CSF) is a hematopoietic growth factor involved in the terminal differentiation and survival of alveolar macrophages with important implications for macrophage-dependent lung physiological responses as well as host protective immunity against pneumotropic bacteria (J Immunol 187: 5346, 2011). Methods: We here performed a comparative analysis of murine bone marrow-derived monocyte (BMM) versus resident alveolar macrophage (rAM) transduction with either lentiviral or adenoviral vectors encoding for murine GM-CSF (LV-GM-CSF versus AdGM-CSF). Results:…