hiPSC derived endothelial cell types from scalable cultures for biofunctionalization and tissue engineering

Introduction: Applications like full endothelialisation of gas exchange membranes in extracorporal membrane oxygenation (ECMO) devices for improved hematocompatibility, cell therapy of pulmonary hypertrophy or tissue engineering require large numbers of (patient-specific) endothelial cells (ECs). The isolation of ECs from peripheral blood or explanted vessels is well established however especially cells from older individuals show a limited proliferation capacity. Patient specific ECs from pluripotent stem cells (hiPSCs) might be an alternative suitable cell source. The opportunity to generate large amounts of u…