Methods to unravel the role of HDAC10 in autophagy-related drug resistance

Autophagy has been described in many malignancies to help advanced cancer cells to cope with stressful conditions such as chemotherapy and radiation leading to an increase in resistance. We have recently identified HDAC10 as a promoter of autophagy-mediated survival in neuroblastoma cells and identified this HDAC family member as a druggable regulator of advanced-stage tumor cell survival. To unravel HDAC10-regulated cell death resistance mechanisms in childhood tumors of the nervous system (medulloblastoma and neuroblastoma) we want to dissect the interplay of apoptosis, necroptosis and autophagy in response to…