Optimization of Glioblastoma multiforme in vitro culture conditions for preservation of EGFR gene amplification

Glioblastoma multiforme (GBM) is the most common and lethal brain tumor. Numerous efforts are made to investigate cellular mechanisms in GBM tumor cells to develop more effective therapies. Hence, optimal in vitro models of GBM are essential, generated from individual patients and analysed at early passages to avoid culture artefacts. Different studies demonstrated that amplification of the egfr gene occurs in many cases of GBM, leading to enhanced activation of the EGFR signalling pathway and thus, driving tumor growth. Although mutations are well maintained in in vitro models of GBM one major cell culture arte…