Systematic identification of novel lincRNAs involved in chemoresistance using CRISPR/Cas9 screens

Einleitung: A large fraction of the human genome consists of non-protein coding genes, including long intergenic non-coding RNAs (lincRNAs). The expression pattern of lincRNAs is specifically altered in different tumor subtypies, indicating an important role of lincRNAs for tumor biology. However, the function of most lincRNAs are poorly characterized so far. Ziele: Systematic identification of lincRNAs that are involved chemoresistance or synthetic lethality towards 5-fluorouracil, oxaliplatin and SN-38 (active metabolite of irinotecan) treatment in colorectal cancer cells. Methodik: CRISPR/Cas9 technology was…