CareLit Fachartikel
The serum protein fetuin-b increases in vitro fertilization rate in mice by inhibiting premature zona pellucida hardening
E Dietzel, J Floehr, E Van de Leur, R Weiskirchen, W Jahnen-Dechent · Geburtshilfe und Frauenheilkunde · 2016 · Heft 10
Dokument
1116053
CareLit-ID
Jahr
2016
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Objective: To prevent premature zona pellucida hardening (ZPH) by fetuin-B supplementation and thus to increase in vitro fertilization (IVF) rate. Materials: recombinant mouse fetuin-B, rmFetuB; metaphase 2 stage oocytes and freshly isolated sperm from C57BL/6 mice. Methods: We fertilized batches of 20 – 30 mouse oocytes in vitro and studied early embryo development until blastocyst hatching. Oocytes were kept with or without rmFetuB during in vitro culture (IVC) and IVF. ZPH was judged by chymotrypsin digestion and by counting the number of attached sperm. Results: In the absence of cumulus cells, rmFetuB dose-…
Schlagworte
After
Cumulus Cells
Chymotrypsin
Blastocyst
Affect
Geburtshilfe und Frauenheilkunde
