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Real-Time Polymerase Chain Reaction (RQ-PCR) for the Monitoring of Epstein-Barr Virus (EBV) Load in Peripheral Blood Mononuclear Cells

H. J. Wagner, W. Jabs, F. Smets, M. Wessel, L. Fischer, G. Offner, H. Kirchner, P. Bucsky · Klinische Pädiatrie · 2000 · Heft 04 · S. 206-210

Dokument
1129741
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Jahr
2000
Publikation
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DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
H. J. Wagner, W. Jabs, F. Smets, M. Wessel, L. Fischer, G. Offner, H. Kirchner, P. Bucsky
Ausgabe
Heft 04 / 2000
Jahrgang 212
Seiten
206-210
Erschienen: 2000-01-01 00:00:00
ISSN
0300-8630
DOI
10.1055/s-2000-9678

Zusammenfassung

Abstract: Measurement of Epstein-Barr virus (EBV) load is useful in peripheral blood for detecting primary and reactivated EBV-infections especially in immunosuppressed patients being at high risk for developing posttransplant lymphoproliferative disorder. For quantification of EBV DNA in peripheral blood of patients two real time polymerase chain reaction (RQ-PCR) assays were developed detecting sequences specific for the BAM HI-W and BAM HI-K region of EBV. In order to determine the optimal material of peripheral blood for RQ PCR analysis, DNA preparations of whole blood, peripheral blood mononuclear cells (PB…

Schlagworte

Epstein-Barr virus posttransplant lymphoproliferative disorder (PTLD) real time quantitative polymerase chain reaction patient monitoring Epstein-Barr-Virus Lymphoproliferation nach Transplantation (PTLD)„Real-Time”-Polymerase-Kettenrea… Patienten-Monitoring Blood Klinische Pädiatrie