Taxol Resistance and its Reversal by Synthetic Isoprenoids in Human Bladder Cancer Cell Line
M. Nakagawa, H. Enokida, T. Gotanda, T. Tachiwada, Y. Imazono, H. Kubo, K. Nishiyama, S. Suzuki, K. Inomata, T. Kishiye · Aktuelle Urologie · 2003 · Heft 04 · S. 250-252
Bibliografische Angaben
Zusammenfassung
Abstract PURPOSE: We investigated the mechanism of action of reversal agents for taxol-resistance in bladder cancers. MATERIALS AND METHODS: We isolated a taxol-resistant cell line (KK47/TX30) from a human KK47 bladder cancer cell line (KK47/WT). We characterized KK47/TX30 cells and screened reversal agents for taxol-resistance. RESULTS: KK47/TX30 cells exhibited approximately 700-fold resistance to taxol and cross-resistance to Vinca alkaloids and topoisomerase II inhibitors. Western blot analysis demonstrated P-glycoprotein (P-gp) overexpression in taxol-resistant cells. Drug accumulation and efflux studies sh…
