Specific expression of the homeobox transcription factor Prox1 in hepatocytes in vitro, and during liver damage and regeneration in vivo
J Dudas, M Papoutsi, M Stefan, D Batusic, A Elmaouhoub, V Cimica, T Pieler, G Ramadori, J Wilting · Zeitschrift für Gastroenterologie · 2004 · Heft 02
Bibliografische Angaben
Zusammenfassung
Aims: Prox1 is one of the earliest markers of the hepatic bud. A 70% reduction of the number of hepatoblasts has been observed in Prox1-/- mice. Prox1 may regulate proliferation, migration and cell-matrix interactions during liver development. Methods: Prox1 expression was determined at RNA and protein levels in whole livers, in cells isolated from rat livers, in met-murine hepatocytes (MMH), and in human hepatoma cells. For mRNA expression, northern blot and real time PCR, for protein expression western blot and immunostaining were used. Animal models: liver damage induced by CCl4 treatment in Wistar rats, part…
