CareLit Fachartikel
Regulated Expression of IFN gamma in the Liver Allows Analysis of the Effect on HBV Replication in Mice
J Dumortier, H Oberwinkler, H Bujard, K Schömig, U Protzer · Zeitschrift für Gastroenterologie · 2004 · Heft 02
Dokument
1131274
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Jahr
2004
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Zusammenfassung
To study antiviral and side effects of antiviral cytokines, we developed an adenoviral vector system that mediates liver specific and regulated cytokine production, and allows to monitor target gene expression. We now used that system, to determine the effect of interferon (IFN) γ on HBV replication in mice. Genes of firefly luciferase and GFP or IFNγ, respectively, were co-expressed under control of a bi-directional promoter Ptetbi in an Ad5ΔE1/E3 vector (Adbiluc). Using the Tet-system, expression of tTA/rtTA under liver specific promoters ensured liver specificity. Double transgenic mice expressing rtTA and HB…
Schlagworte
Hepatitis B
Zytokin-Therapie
Cytokine
Cytokines
Bread
After
Zeitschrift für Gastroenterologie
