Differences between somatic (in-site biopsies) and germ-line (peripheral blood) mutations in gastrointestinal diseases

Aims: Research protocols for the detection of disease related DNA polymorphisms is concentrating around germ-line alterations detectable in peripheral blood samples. Less is known about polymorphisms in the DNA isolated from the altered tissue biopsy. Materials and methods: After informed consent, peripheral blood was drawn and gastric biopsy samples were taken from the pathological and healthy part of the stomach and duodenum. Genomic DNA was extracted from biopsy and blood samples from 15 patients suffered from intestinal metaplasia, 15 from ulcus duodeni, 15 from gastritis, 15 from gastric cancer, 15 from atr…