Combined use of 4-color flow cytometry and real-time PCR to detect minimal residual disease (MRD) in childhood acute lymphoblastic leukemia
G Kerst, H Kreyenberg, E Koscielniak, G Dückers, C Niemeyer, I Müller, K Schilbach, R Handgretinger, PG Schlegel, D Niethammer, P Bader · Klinische Pädiatrie · 2004 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: MRD detection in ALL patients is commonly performed by PCR amplification of clone-specific immunoglobulin and T-cell receptor gene rearrangements. Despite its high sensitivity, PCR amplification carries the risk of false-negative results due to clonal evolution and can not yet be applied to all patients. Therefore, the additional use of a second method seems feasible. Methods: Flow cytometric analysis of MRD was performed according to the protocols invented by E. Coustan-Smith and D. Campana (St. Jude Children's Research Hospital, Memphis, TN). Real-time PCR was performed by the use of Taqman® tech…
