CareLit Fachartikel
Inwardly Rectifying K+ Channel (Kir)–4.1 Localizes to Tubulovesicles and Secretory Membranes from resting and stimulated rat stomach
MA Kaufhold, A Krabbenhöft, M Fährmann, M Manns, U Seidler, S Hagen · Zeitschrift für Gastroenterologie · 2004 · Heft 08
Dokument
1131848
CareLit-ID
Jahr
2004
Publikation
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Metadaten
DOI
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Zusammenfassung
Although it is well established that K+-recycling occurs during gastric acid secretion, the identity of the channel involved in this process has been controversial. This is because it is difficult to determine conclusively, by immunostaining alone, whether putitive K+-channel proteins reside on the apical membrane or in tubulovesicles in resting parietal cells. Here we show, using purified membrane fractions, that Kir4.1 is very closely associated with the H/K ATPAse in resting and stimulated parietal cells. METHODS: Membrane fractions were isolated from rat stomach that had been either maximally stimulated to s…
Schlagworte
Kir4.1
membrane fractionation technique
rat gastric acid secretion
ATPase
Association
Zeitschrift für Gastroenterologie
