Global Analysis of Host Tissue Gene Expression in the Invasive Front of Colorectal Liver Metastases
K Brand, OR Bandapalli, C Franke, D Kobelt, K Kühnle, S Elezkurtaj, J Herrmann, AM Gressner, R Weiskirchen, D Beule, N Blüthgen, H Herzel, M Geheeb · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Bibliografische Angaben
Zusammenfassung
Host cell reactions are a crucial determinant for tumorous invasion. We analysed on a genome wide scale gene expression differences between microdissected tissues taken from unaffected liver tissue of a human colorectal tumor (LS174) growing in the livers of nude mice and tissue from the host part of the invasive front. Due to the low degree of interspecies cross hybridization of 15% as determined on Affymetrix microarrays, our xenograft model allowed for the distinction of genes of murine versus human origin even if the respective tissues could not be isolated separately. With this type of model the reaction of…
