Prediction of Metabolic Functions of Primary Human Liver Cells Maintained in Perfused 3D Multi-Compartment Bioreactors for Extracorporeal Liver Support
K Zeilinger, M Pfaff, R Guthke, W Schmidt-Heck, S Töpfer, D Woetzel, D Driesch, G Pless, P Neuhaus, J Gerlach · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Bibliografische Angaben
Zusammenfassung
Aims: The development of a bioreactor providing a three-dimensional network of interwoven capillary membranes with integrated oxygenation and decentralized mass exchange enables the culture of primary liver cells under conditions closely reflecting the natural environment of the cells in vivo (1,2). In order to allow an early estimation of the quality of bioreactor cultures intended for clinical application, the determination of predictive parameters allowing the prognosis of the long-term culture performance is of interest. Methods: Human liver cells were obtained from 18 human livers discarded from the liver t…
