Detection of magnetically labelled embryonic stem cells in the rat brain by high resolution MR-Imaging in vivo
C Sieland, A Stroh, C Faber, T Neuberger, K Weir, K Hild, S Mueller, P Lorenz, T Grune, P Jakob, M Taupitz, H Pilgrimm, J Schnorr, C Zimmer · RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren · 2005 · Heft 02
Bibliografische Angaben
Zusammenfassung
Objectives: Stem cell transplantation is a promising approach in the therapy of Parkinson's Disease (PD). We used high resoltution MRI together with an effective magnetic labelling to asses the detection limit, localisation and migration of transplanted embryonic stem cells in a rat model of PD. Additionally the influence of the magnetic label on the biology of the cells was investigated. Materials and Methods: Murine embryonic stem cells were magnetically labelled in vitro with Very Small Super-Paramagnetic Iron-Oxide-Particles (VSOP). The lipofection agent FuGENE was added in different concentrations during in…