cAMP-dependent inhibition of surface cell NHE3 and mucosal PDZ-domain binding protein expression levels are reduced in CFTR deficient murine colon

cAMP-dependent inhibition of electroneutral NaCl-absorption in the intestine by Na+/H+ exchange is regulated by a multiprotein complex consisting of transport and adapter proteins, cytoskeletal structures, and protein kinases, and represents a central pathomechanism in secretory diarrhea. This inhibition is lacking in intact sheets of intestinal mucosa from CFTR-deficient mice. Studies in renal and pancreatic cell lines and epithelia have identified adapter proteins that are required, but evidence in native intestinal cells is sparse. To search for the molecular mechanisms of the lack of cAMP-dependent inhibitio…