Measurement of intracellular pH: differences between microspectrofluorimetric and imaging systems
P Hegyi, V Venglovecz, I Ignáth, B Ózsvári, T Takács, J Lonovics, A Varró, Z Ifj Rakonczay · Zeitschrift für Gastroenterologie · 2006 · Heft 06
Bibliografische Angaben
Zusammenfassung
Background. Since 1979, more than a thousand papers have reported the use of biscarboxyethylcarboxyfluorescein (BCECF) for making measurement of intracellular pH (pHi) using microspectrofluorimetric systems (MSFS). Looking critically at these papers, some odd and unexplained things can be observed. For example, there are big differences in the resting pHi of the cells, even in similar cells, in similar conditions and described in the same paper. Despite the huge development of imaging systems (IS), only few imaging measurements can be found in the literature to monitor real-time changes of pHi. Our aim was to co…
