EGFR reactivates telomerase in immortalized squamous epithelial cells through PI3K/AKT-mediated phosphorylation and transcriptional regulation via Hif-1α
S Heeg, M Quante, E Scheffer, A von Werder, M Döbele, G Gössel, C Fulda, H Kunert, H Nakagawa, R Beijersbergen, HE Blum, OG Opitz · Zeitschrift für Gastroenterologie · 2006 · Heft 09
Bibliografische Angaben
Zusammenfassung
Introduction: In our cellular model of esophageal carcinogenesis immortalization was induced independent of telomerase but through alternative lengthening of telomeres (ALT). In this stepwise model additional overexpression of EGFR in immortalized cyclin D1 overexpressing/p53 inactivated cells (OKF6 D1/del.p53) led to in vitro transformation and activation of telomerase. This study identifies the cellular pathways involved in the activation of telomerase and in vitro transformation induced by EGFR. Methods: We overexpressed EGFR in immortalized OKF6 D1/del.p53 cells by retroviral mediated gene transfer. Expressi…
