Increased wound-response revealed by proteomics of estrogen receptor-negative breast cancers

Besides the mitogenic action of estrogens in breast cancer cells there is also evidence that estrogen receptors mediate protective, anti-invasive effects. The aim of this project was to identify downstream ER-dependent proteins differentially expressed in fresh-frozen ER-positive and –negative breast cancer. Tissue sections from eight ER-positive and eight ER-negative cryo-preserved breast cancer specimen were analysed by differential multiplex ProteoTope imaging of 2D-PAGE gels. This method is based on labeling proteins with chemically identical iodine radio-isotopes (I–125, I–131) and provides a direct quantit…