Excitatory actions of supernatants released from mucosal biopsies of IBS patients is mediated by proteases, serotonin and histamine
S Buhner, Q Li, S Vignali, R Langer, G Barbara, R De Giorgio, V Stanghellini, F Zeller, K Michel, M Schemann · Zeitschrift für Gastroenterologie · 2008 · Heft 09
Bibliografische Angaben
Zusammenfassung
Einleitung: Irritable bowel syndrome (IBS) symptoms may be linked to altered mediator release from the gut epithelium. We previously reported that supernatants released from mucosal biopsies of D- and C-IBS patients activate human enteric neurons. Ziele: The aim of the present study was to pharmacologically identify the active components in the supernatants. Methodik: We used a fast neuroimaging technique with a voltage sensitive dye (DI-8-ANEPPS) to record activity in human enteric neurons after local application of IBS mediator cocktail. Ergebnis: Application of all IBS mediator cocktails (7 D-IBS and 4 C-IBS)…
