CareLit Fachartikel
Independent enzymatic control of oxygen and H2O2 in cultured cells
G Millonig, GN Waite, S Hegedüsch, HK Seitz, S Mueller · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Dokument
1135126
CareLit-ID
Jahr
2009
Publikation
PDF
nein
Metadaten
DOI
ja
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Bibliografische Angaben
Zusammenfassung
Aims: Oxidative stress has been identified as a major pathogenic factor in many liver diseases including alcoholic and non-alcoholic liver disease, hereditary hemochromatosis, and chronic hepatitis C. To mimic such conditions in vitro, oxidants such as H2O2 or redox-cycling drugs such as doxycyclin or antimycin A are routinely applied to cultured cells. However, these experiments are typically performed at air oxygen levels of 21%. This is hyperoxic as compared to the normal tissue environment considered to be at ca. 1–2% O2. We here aimed at developing an enzymatic system that allows to independently control ox…
Schlagworte
cell culture system
enzyme
hypoxia
oxidative stress
Antimycin A
Diffusion
Hepatitis
Zeitschrift für Gastroenterologie
