CareLit Fachartikel
The novel histonedeacetylase inhibitor Panobinostat induces alternative pathways of apoptosis in hepatocellular carcinoma models
P Di Fazio, R Schneider-Stock, T Wissniowski, S Gahr, D Neureiter, M Ocker · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Dokument
1135129
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Jahr
2009
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Zusammenfassung
Aims: Inhibitors of histone deacetylases (HDACi) represent a novel therapeutic option for human cancer diseases. The novel pan-HDACi Panobinostat (LBH589) induces apoptosis in human hepatocellular tumor cells in vitro and in xenograft models in vivo. Here, we analysed the underlying signalling pathways in more detail. Methods: Human hepatocellular carcinoma (HCC) cell lines HepG2 (p53wt) and Hep3b (p53null) were cultured under standard conditions and treated with 0.1 or 0.01µM Panobinostat. Apoptosis was quantified by flow cytometry and verified by immunofluorescence of cytokeratin 18 cleavage. Mitochondrial tra…
Schlagworte
HDAC
UPR
apoptosis
miRNA
p53
Caspase 8
Tumor
Caspase 12
Caspase 3
Caspases
Zeitschrift für Gastroenterologie
