Trypsin inhibits a chloride independent pancreatic ductal bicarbonate secretion via protease-activated receptor 2

Background: Low doses of ethanol and bile acids stimulate pancreatic ductal bicarbonate secretion, whereas in high concentrations they will cause global inhibition of ion transport. Aim: To investigate the effects of trypsin on pancreatic duct cells. Methods: Pancreatic protease-activated receptor 2 (PAR2) expression was determined by immunohistochemistry. Intracellular pH (pHi) and Ca2+ concentration ([Ca2+]i) were measured on isolated intra/interlobular microperfused guinea pig pancreatic ducts by microfluorimetry. Results: PAR2 expression was found on the luminal membrane of intralobular, but not on interlobu…