GABA outflow is modulated by electrical high frequency pulses during N-methyl-D-aspartate receptor activation in the rat caudate nucleus

GABAergic neurons have been demonstrated to play an essential role in the mechanism of high frequency stimulation of deep brain regions, which is an effective treatment in Parkinson's disease. In this study, the effects of electrical high frequency (hf)-pulses and N-methyl-D-aspartate (NMDA) receptor activation on GABA and glutamate outflow were examined in slices of the rat caudate nucleus. Therefore, superfusion experiments were performed and GABA and glutamate outflow were measured by means of HPLC with electrochemical detection after pre-column derivatisation with o-phthaldialdehyde sulphite. After a 40min w…