The role of the selenoprotein S (SELS) gene -105G>A promoter polymorphism in inflammatory bowel disease and regulation of SELS gene expression in intestinal inflammation
J Seiderer, J Dambacher, B Kühnlein, S Pfennig, A Konrad, D Haller, B Göke, T Ochsenkühn, P Lohse, S Brand · Zeitschrift für Gastroenterologie · 2007 · Heft 09
Bibliografische Angaben
Zusammenfassung
Background/aim: Recently, a -105G>A promoter polymorphism coding for selenoprotein S (SELS) has been shown to increase proinflammatory cytokine expression (Nature Genetics 2005; 37:1234–41). We therefore analyzed SELS expression and potential phenotypic consequences of the -105G>A polymorphism in patients with inflammatory bowel disease (IBD). Methods: SELS mRNA was measured by quantitative PCR in intestinal epithelial cells (IEC) after stimulation with proinflammatory cytokines and in human colonic biopsies of IBD patients as well as in the TNFdeltaARE mouse model of ileitis. Genomic DNA from 563 individuals (C…
