Screening for HCV-specific T cell responses: THE USE OF PEPTIDE POOLS IMPAIR THE PROLIFERATION OF ANTIGEN SPECIFIC CD8 T-CELLS
PV Suneetha, V Schlaphoff, C Wang, SK Sarin, MP Manns, M Cornberg, H Wedemeyer · Zeitschrift für Gastroenterologie · 2008 · Heft 01
Bibliografische Angaben
Zusammenfassung
Proliferation of antigen-specific CD4/CD8 T-cells, in response to stimulation using peptide pools, has been routinely used in epitope discovery and vaccine development research. However, neither the optimal concentration of peptides nor the number of peptides per pool has been defined. Thus, the main objective of this study was to examine whether the number of peptides per pool or different peptide concentrations influence different effector functions of T-cell responses against defined CD8 T-cell epitopes. For this purpose, PBMCs isolated from HLA-A2-positive individuals responding to either to the influenza ma…
