The tuberculin skin test in mice mainly depends on interferon-gamma-producing CD4+ T- lymphocytes
C Hölscher, A Hölscher, T Schreiber, D Rückerl, P Adams, D Woodland, A McKenzie, F Brombacher, Y Iwakura, S Ehlers, C Lange · Pneumologie · 2008 · Heft 04
Bibliografische Angaben
Zusammenfassung
The tuberculin skin test (TST) reaction depends on the presence and function of T lymphocytes. However it is still unclear whether numbers of T-cells or their ability to secrete interferon-gamma (IFN-g) are key to determine a TST reaction. Defined numbers of purified CD4+ T-cells were isolated from Mycobacterium tuberculosis (Mtb)-infected wildtype mice and transferred into alymphoid gamma-c-RAG2-deficient mice. The antigen specificity and the ability to sectete IFN-g of transferred cells were verified by staining with IAb ESAT6(1–20) multimers and by an IFN-g ELISPOT assay. After subcutaneous injection of PPD i…
