CareLit Fachartikel
Role of ERK1/2/MAPK and cFLIP Signalling in Apoptosis Suppression by Ursodeoxycholyl Lysophosphatidylethanolamide (UDCA-LPE)
W Chamulitrat, A Pathil, W Stremmel · Zeitschrift für Gastroenterologie · 2010 · Heft 01
Dokument
1138267
CareLit-ID
Jahr
2010
Publikation
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Metadaten
DOI
ja
zitierfähig
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Zusammenfassung
We had synthesized the bile acid-phospholipid conjugate UDCA-LPE which was demonstrated to be cytoprotective against TNF-α-induced apoptosis with a mechanism involving cFLIP/caspase 8 signalling. We found that UDCA-LPE activated ERK1/2 phosphorylation. It is known that ERK1/2/MAPK pathway regulates cellular survival and apoptosis, we now ask a question whether ERK1/2 could regulate cFLIP during intrinsic apoptosis. HepG2 cells were subjected to knockdown experiments using cFLIP, ERK1/2 or control siRNAs, and subsequently treated with UDCA-LPE or vehicle. cFLIP mRNA and protein was analyzed by RT-PCR and Western…
Schlagworte
apoptosis
bile acid-phospholipid conjugate
liver disease
Caspase 8
Caspase 3
Zeitschrift für Gastroenterologie
