Synchrotron-based Micro-CT Imaging of the Human Lung Acinus
R Moritz, AC Langheinrich, HD Litzlbauer, K Korbel, TL Kline, SM Jorgensen, DR Eaker, RM Bohle, EL Ritman · RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren · 2010 · Heft 11
Bibliografische Angaben
Zusammenfassung
Aim: Structural data about the human lung fine structure are mainly based on stereological methods applied to serial sections. The purpose of our study was to generate a complete data set of the intact 3-dimensional architecture of the human acinus using high-resolution synchrotron-based micro-CT (synMCT). Material and Methods: A human lung was inflation-fixed by formaldehyde ventilation and then scanned in a 64-slice CT over its apex to base extent. Lung samples (8-mm diameter, 10-mm height, n=12) were punched out, stained with osmium tetroxide, and scanned using synMCT at (4µm)3 voxel size. The lung functional…
