Separation of methylated CFDNA fraction with methyl miner method

Introduction: During the progression of colorectal cancer the circulating cell free DNA (cfDNA) level is increasing in the plasma from 10–20ng/ml to 100–200ng/ml or more. The examination of the plasma cfDNA can help us to understand the alteration of methylation profile like genereal hypomethylation and local hypermethylation in colorectal cancer. Aims: Our aim has been separation, quantification and examination of the methylated and non-methylated cfDNA fractions from peripheral blood of cancer patients. Materials and methods: We have been isolating the cfDNA from plasma with QIAamp Circulating Nucleic Acid Kit…