Evaluation of single nucleotide polymorphisms as internal controls in prenatal diagnosis of fetal blood groups

Ziel: Determination of fetal blood groups in plasma of pregnant women is increasingly utilized as an additional diagnostic method. However, test interpretation can fail, if insufficient amplification of fetal DNA occurs (a negative test result is expected, if the fetus is negative but may also occur if the amplification of the fetal DNA fails). To discriminate fetal and maternal DNA we evaluated the use of 52 SNPs as individual internal positive controls (IPC) in our PCR settings for prenatal blood group typing. Methodik: DNA from 224 plasma samples (fetal & maternal DNA) was screened for 52 SNPs divided into fo…