CareLit Fachartikel
HCV/TLR3 modulated the expression of RANTES in murine activated hepatic stellate cells
B Wang, R Bröring, CI Real, M Trippler, G Gerken, M Lu, JF Schlaak · Zeitschrift für Gastroenterologie · 2012 · Heft 01
Dokument
1140465
CareLit-ID
Jahr
2012
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Background/Aims: RANTES/CCL5 has been identified as a central mediator in liver fibrogenesis. However, the interaction between HCV/TLR3 and RANTES and its functional role for hepatic stellate cells (HSC) is not well understood. Methods: Wide-type and TLR3-/- mice were treated with TLR3 ligands (Poly I: C). The expression of hepatic RANTES and IFN-β was detected by quantitative real time RT-PCR (qrt-PCR). Primary HSCs were isolated from human liver tissues or C57BL/6J wild-type mice. HSCs were stimulated with different TLR ligands for 20h. RANTES mRNA expression and protein production were assessed by qrt-PCR and…
Schlagworte
HCV
Hepatische Sternzellen
RANTES
TLR-System
Expression
Could
Hepatic
Cells
Liver
Qrt-Pcr
Genes
Murine
Activated
Stellate
Functional
Zeitschrift für Gastroenterologie
