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Application of upcyte® technology to primary cells for developing alternatives to current in vitro metabolism models

A Nörenberg, C Daehn, K Scheller, N Hewitt, S Heinz, J Braspenning · Zeitschrift für Gastroenterologie · 2012 · Heft 01

Dokument
1140468
CareLit-ID
Jahr
2012
Publikation
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DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
A Nörenberg, C Daehn, K Scheller, N Hewitt, S Heinz, J Braspenning
Ausgabe
Heft 01 / 2012
Seiten
–
Erschienen: 2012-01-09 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0031-1295818

Zusammenfassung

We have developed a new technology to allow the proliferation of differentiated primary cells without inducing permanent immortalization, uncontrolled cell growth, or loss of phenotype. The Upcyte® (“upregulated”) technology involves a viral gene transfer system to introduce a unique combination of genes that induce and maintain cell proliferation until the cells reach confluence. This allows the primary cells to be passaged many times with the generation of billions of cells. Upcyte® technology can be applied to different cell types such as hepatocytes. Here, we summarise some of the comparisons we have made be…

Schlagworte

ADMET hepatocyte in vitro metabolism upcyte hepatocytes Upcyte Cells Technology Primary Hepatocytes Vitro Different Application Allows Donors Assays