CareLit Fachartikel
In vivo RNAi screening to genetically dissect malignant progression from hepatocytes harboring constitutive active AKT–1
A Hohmeyer, D Dauch, TW Kang, T Longerich, P Schirmacher, M Manns, L Zender · Zeitschrift für Gastroenterologie · 2012 · Heft 01
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1140484
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2012
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Zusammenfassung
The neoplastic evolution of hepatocellular carcinoma (HCC) proceeds through a multi-step process which is still poorly characterized on the molecular level. The discovery of RNA interference (RNAi) has revolutionized loss of function genetics and RNAi based functional genomic screens allow for the identification of unbiased and comprehensive collections of new gene targets in virtually every biological system. Through the combination of intraheptic gene transfer via transposable elements with miRNA based shRNA technology, we developed a system allowing to conduct in vivo RNAi screens for new tumor suppressor gen…
Schlagworte
AKT-1
HCC
RNAi
deep sequencing
in vivo screen
Tumor
Zeitschrift für Gastroenterologie
