Lentiviralmarking as a tool to investigate the clonal complexity and evolution of ALL

Introduction: There remains significant controversy over the applicability of cancer stem cell models to acute lymphoblastic leukaemia. Recent data suggest that the propagating compartment undergoes a clonal evolution process, leading to the co-existence of several different subclones. Methods: We aim to provide a comprehensive picture of the clonal complexity and evolution of ALL by lentivirally marking individual leukaemia cells with heritable genetic markers. This will be achieved using a cellular barcoding strategy and analysis of lentiviral insertion sites. This will allow us to address numerous questions,…