Searching for the molecular benchmark of intestinal anastomotic healing in rats: an experimental study

Objective: Intestinal anastomotic healing processes differ greatly from analogous more widely-researched cutaneous phenomena. To date, we still lack comprehension of the particularities of the physiology of intestinal healing. Here, we examine the mRNA response time course to formation of an anastomosis. Materials and methods: In 8 rats, end-end ilio-ilial anastomoses were performed (n=2 per group). At days (d) 0 (control), 2, 4 and 8 10mm anastomotic segments were resected and examined by Affymetrix microarray (RG-230; >31000 probe sets) to assess changes in gene regulation. In addition to seeing the global tim…