Influence of USP2-Isoforms on TNF-a dependent NFkB activation in primary hepatocytes versus hepatoma cells

Backgrounds and aims: Ubiquitination and deubiquitination of proteins have diverse functions within the cell. Among others, both are involved in regulation of signalling pathways. Within the Tumour necrosis factor (TNF)-a dependent NFkB signalling pathway multiple ubiquitination and deubiquitination steps occur to induce cell proliferation and survival but TNF-a also triggers apoptosis. The deubiquitinating enzyme Ubiquitin-specific protease 2 (USP2) seems to play an important role within this pathway. Two different USP2 isoforms exists, a shorter USP2–41kDa and a longer USP2–69kD isoform. We analysed both USP2…