Evaluation of an automated hyaluronan latex agglutination assay enables the implementation of routine laboratory HEPASCORE calculation

Background: The non-invasive HEPASCORE was reported as a diagnostic tool for predicting liver fibrosis. To date, hyaluronan (HA) is still predominantly assayed by time-consuming and costly enzyme-linked protein binding assay (ELISA). Methods: We aimed to evaluate the analytical performance of a novel automated HA-latex agglutination assay (WAKO, Osaka, Japan) using KoneLab 30i analyzer (ThermoFisher Scientific, Hudson, New Hampshire, USA) and compared HA-concentrations in healthy blood donors (N=57) to the reference HEPASCORE-ELISA (HA Test Kit, Corgenix, Westminster, USA). Results: The lowest detectable level o…