The p53 Family Gene Signature Predicts Prognosis in Hepatocellular Carcinoma
F Staib, A Pelc, S Eftekhar-Afzali, M Krupp, SS Thorgeirsson, PR Galle, T Schilling, XW Wang, A Teufel, M Müller · Zeitschrift für Gastroenterologie · 2013 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: We have previously established a key role for p53, TAp63, and TAp73 in hepatocarcinogenesis. We now performed a large scale screening for p53 family target genes and their interacting networks. Methods: rAd-p53, rAd-TAp63 or rAd-TAp73 were transfected in Hep3B cells. RNA was isolated ofter 24 and 36 hours and hybridized against customized microarrays. Resulting genetic signatures were tested for prognostic relevance using unsupervised clustering, Kaplan-Meyer Analysis and survival risk prediction. Results: We identified multiple novel potential p53, pTA63, and pTA73 target genes. The p53-, TAp63- and…
