Xenologous engraftment of juvenile myelomonocytic leukemia in BalbC/Rag2null/γcnull mice

Juvenile myelomonocytic leukemia (JMML) is a malignant myeloproliferative disorder of early childhood. Mutations of the Ras signaling pathway are present in 80% of patients but do not fully explain the heterogeneity observed clinically. Epigenetic modifications are important determinants of resistance to therapy. To improve our understanding of epigenetics in JMML leukemic stem cell biology, we established a xenotransplantation model of primary human JMML cells into BalbC/Rag2null/γcnull mice. Intrahepatic injection of 8*105 cells into 2-day-old mice after irradiation with 2.5 Gray resulted in durable engraftmen…