Different activating effect of genomic DNA from normal and tumorous epithelial cells on HT-29 carcinoma cells, HDF-Alpha fibroblasts and peripheral mononuclear blood cells
I Fűri, F Sipos, S Spisák, Á Patai, B Wichmann, G Műzes, O Galamb, G Valcz, A Kalmár, K Leiszter, K Tóth, B Barták, Z Nagy, B Molnár, Z Tulassay · Zeitschrift für Gastroenterologie · 2013 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: The methylation pattern of genomic DNA fragments may represent its organ origin. In case of tumorous and inflammatory conditions, the amount of cell-originated DNA is elevated in the serum. The mediation and immunobiologic effect of methylated DNA sequences on Toll-like receptor 9 expressing cells are not fully understood. Aims: Our aims were to examine and compare the TLR9- and pro-inflammatory cytokine associated immunobiologic effect of human DNA from normal- and tumor origin on different cell types and ex vivo peripheral mononuclear cells (PBMCs). Methods: Genomic DNA was isolated from fresh froz…
