Pathway analysis of membrane-initiated effects of estrogen-norethisterone (E-NET) treated breast cancer cells overexpressing PGRMC1 in a mouse model

Progesterone receptor membrane component-1 (PGRMC1) is overexpressed in breast cancer and it may contribute to tumorigenesis during hormone therapy. In this study we established a method for protein extraction from FFPE tissues to set up reverse phase protein microarray (RPPM) technology and to analyze expression and phosphorylation of PGRMC1-signaling associated proteins in tumor tissues obtained in a transplantation model (Neubauer et al., 2013). Different methods for target retrieval such as the usage of target retrieval solution (TRS, DAKO) or citrate buffer combined with various protein extraction methods s…