MeV-RII_HepG2, a persistently measles virus (MeV) infected human hepatoma cell line, displays resistance towards secondary infections with MeV virotherapeutics

Introduction: The oncolytic potential of measles vaccine virus (MeV) has been shown both in vitro and in vivo. On the contrary, resistances to MeV-mediated oncolysis were demonstrated to either pre-exist or arise in the course of MeV virotherapy. To investigate mechanisms of secondary resistance we have generated a hepatoma cell line being persistently infected with MeV (MeV-RII_HepG2) which was generated by two rounds of infection with the GFP reporter gene encoding vector MeV-GFP followed by expansion of surviving (resistant) cell clones. Aims: To characterize phenomena of secondary resistance to MeV-based vir…