Long-term maintenance of human liver tissue by ex-vivo perfusion of liver sections
T Schreiter, J Sowa, Z Mathe, J Treckmann, M Broecker-Preuß, H Baba, RK Gieseler, G Gerken, A Canbay · Zeitschrift für Gastroenterologie · 2014 · Heft 01
Bibliografische Angaben
Zusammenfassung
Introduction: We recently presented a perfusion system for human liver sections able to keep hepatic functionality for six hours and to reflect clinical parameters of patients in non-cirrhotic and cirrhotic tissue (1). Here we demonstrate the successful extension of the perfusion time for up to 24 hours. Methods: The initial setup of the perfusion system was modified concerning medium composition, oxygen supply and pH adjustment. Hourly taken samples from the perfusate were analyzed for markers of general metabolism, hepatic synthesis capacity and liver cell damage. Results: The crucial phase of the perfusion ap…
