Incidence of EGFR, KRAS, BRAF, ALK, and p53 alterations in a cohort of 161 consecutively tested patients from a certified lung cancer center, correlation with clinical characteris…

Introduction: The aim of the study was to systematically analyze EGFR, KRAS, BRAF, ALK and p53 within the same cohort of patients, to correlate alterations with clinical characteristics and to potentially identify new prognostic/predictive markers. Methods: 161 patients from a single center diagnosed with lung cancer were studied for the presence of EGFR mutations, ALK rearrangements, KRAS codon 12/13/61 and BRAF V600E and G469A, as well as inactivating p53 mutations. Methods for the detection of EGFR mutations included Sanger Sequencing and hybridization based COBAS testing. ALK rearrangements were either teste…