Imbalance of rare CCR10+ CD8+ and CD4+ memory T cells in children at onset of type 1 diabetes
B Upadhyaya, D Kuehn, S Ruhwald, M Bonsack, M Krassner, K Warnke, A Gavrisan, AG Ziegler, E Bonifacio · Diabetologie und Stoffwechsel · 2014 · Heft 05
Bibliografische Angaben
Zusammenfassung
Type 1 diabetes is a chronic autoimmune disease characterised by the presence of memory autoreactive CD4+ and CD8+ T cells that mediate islet beta cell destruction. We asked whether there are changes in the distributions of major and rare memory T cell subsets in the children with type 1 diabetes. Peripheral blood samples from 47 children at diabetes onset, aged 7 – 11 years, and from 47 age-matched autoantibody negative control children were processed for multi-parameter FACS analysis for markers of memory lymphocytes and the surface markers CD25, CCR7, CXCR5, CCR4, CCR10, CRTH2, CCR6 and CXCR3. Boolean gating…
