Phosphoproteomic Analysis of Glioblastoma Reveals Distinct Molecular Subclasses Defined by CREB, NOTCH1 and GSK3b and Different Overall Survival

Introduction: Malignant gliomas, especially glioblastoma multiforme (WHO grade IV), are largely heterogeneous tumor entities. Here, we intended to perform a selective phosphoproteomic analysis and correlate it to clinical outcome and standard molecular subclassifications based on published microarray data. Methods: More than 60 tumor and control biopsies from tumor center and periphery (based on neuronavigation) were subjected to selected phosphoprotein analysis using reverse-phase protein arrays incubated with selected antibodies against an array of posttranslationally modified cancer pathway proteins. The rati…