Mouse hepatic stellate cells are responsive to LPS and contribute to the acute phase response of hepatocytes
R Liebe, K Breitkopf-Heinlein, K Waldow, S Braun, M Thomas, F Schildberg, P Knolle, U Zanger, U Klingmüller, M Ebert, S Dooley · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: The acute phase response (APR) is a systemic inflammatory condition initiated by activated phagocytic cells, fibroblasts and endothelial cells to prevent tissue damage and initiate repair processes. The aim of our study was to quantify the contribution of hepatic stellate cells (HSC) towards the APR induction. To this end, we quantified the secretory output of HSC with respect to the main acute phase cytokines. Hepatocytes' response to the secretory HSC output was analysed by Fluidigm real time PCR analysis. Methodology: Primary HSC were cultivated for 48 hours before adding LPS to the medium. The re…
