A quantitative metabolomic profiling and corresponding modeling approach of hepatocytes with activated Wnt/ß-Catenin signaling
J Böttger, W Schmidt-Heck, M Matz-Soja, C Thiel, U Hofmann, S Vlaic, R Guthke, R Gebhardt · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
The maintenance of liver homeostasis relies on the metabolic zonation, where pericentral (pc) and periportal (pp) zones differ in their enzyme composition, content and activity. To carry out a metabolic fingerprint along the porto-central axis of the sinusoid is of high relevance for establishing a suitable systems biology approach aiding a better mechanistic understanding of hepatocytes heterogeneity with their different cellular functions. Here, we present a metabolic modeling approach for mouse hepatocytes based on metabolite profiles in the cell and cell culture medium of of APCloxPneo mice, with an over-act…
